The final step in entering data into BayesFold is to provide chemical mappings if you have any. The input screen is shown below:
To enter a chemical mapping:
Select the chemical name.
Enter the concentration of the chemical, with its units.
Select the sequence on which this mapping was done.
Enter the start base of the mapping. This should be an integer greater than zero, calculated relative to the first base of the 5' primer (the base with position 1).
Enter the mapping data. These data are raw numbers from integration of radioactive signal in each band, and are proportional to the intensity of modification. The program will automatically rescale them internally onto a 0-to-1 scale.
Data should be entered as a list containing a number for each base for which data is known, separated by white space (spaces and/or tabs). The list must be contiguous, so any positions without mapping data must be represented in the list by an "x" except for gaps, which should not be included in the list. For example, the following is a sample list: 12 1.4 x 3 4 x x x 2.
Note that mapping data may be pasted in directly from Excel if they are held in a row. If the data are not in a row in Excel, you can easily put them in one by copying their column and then using "Paste Special" with the "Transpose" option checked to paste them into a row.
Repeat the above steps for up to five chemical mappings. Then click the "Validate" button to identify any errors. Correct and revalidate the input as necessary; when the "Continue" button becomes active, click it to submit your data for processing.
If you want to start over, click the Clear button to discard all the data you have entered since step 1 and begin again.